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If you use smart to explore domain architectures, or want to find exact domain counts in various genomes, consider switching to genomic mode. 今天为大家介绍一个预测蛋白结构域的数据库：SMART (Simple Modular Architecture Research Tool)，它是一个用于蛋白质结构域鉴定、注释的在线分析工具。 在文本框中输入蛋白序列（可以只有氨基酸序列，也可以以fasta格式粘贴），把下面的要鉴定的复选框都选上，点击“Sequence SMART”就可以进行蛋白结构域分析了，速度非常快。 SMART（http://smart.embl.de/）是我们常用来 进行domain搜索和蛋白质注释的数据库， 它集成了很多蛋白结构预测和功能分析的工具，比如可以预测蛋白的一些二级结构：跨膜区（Transmembrane segments）、复合螺旋区（coiled coil regions）、信号肽（Signal peptides）、蛋白结构域.
SMART数据库，最新版v8，收录超1300蛋白结构域信息，覆盖uniprot、ensembl等数据库。 提供正常及基因组模式，支持蛋白ID和结构域检索，展示结构域图、蛋白相互作用、通路信息、同源群注释及转录后修饰。 通常分析结构域用到的数据库有pfam、NCBI CDD和SMART。 本文记录一下通过TBTools一键分析基因结构域并利用工具可视化的具体步骤。 SMART（Simple Modular Architecture Research Tool）是一款用于识别、注释和分析蛋白质结构域的工具，包含500多个结构域家族，主要集中于信号传导、细胞外和染色质相关蛋白。 Smart (a s imple m odular a rchitecture r esearch t ool) allows the identification and annotation of genetically mobile domains and the analysis of domain architectures
文章介绍了如何使用在线工具SMART对蛋白序列进行结构域分析的基本步骤,从输入序列、选择数据库与设置,到分析结果和进一步的研究.
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